Additional file 2.
Figure showing the nuclear factor kappa B (NF-κB) pathway and Hes1 activity are needed for the survival of neurons, while transforming growth factor β1 (TGFβ1) is unable to rescue cells from death. E17 hippocampal neurons were plated at a density of 30,000 cells/cm2 and cultured for 7 days in vitro (DIV). Neurons were (A) treated for 24 h with SN-50 (5 μM) or with its control peptide in the presence or absence of TGFβ1 (10 ng/ml). (B) Neurons were co-transfected with enhanced green fluorescent protein (EGFP) and a myc-tagged Hes6 vector for 48 h in the presence or absence of TGFβ1. The cells were fixed and labeled with anti-EGFP and anti-myc antibodies, while the integrity of their nuclei was assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Note that the obliteration of either NF-κB activation or Hes1 activity was followed by neuron death. The addition of TGFβ1 did not reverse these effects.
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Chacón and Rodríguez-Tébar Alzheimer's Research & Therapy 2012 4:31 doi:10.1186/alzrt134